Human IRF5 antibody

Interferon regulatory factor 5 (IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs (IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.

Product Specifications

Product Name Alternative

Interferon regulatory factor 5, irf5

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IRF-5 (176-240aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

10T1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IRF5, ATGA0518-10 µg, ATGA0518-20 µg, ATGA0518-50 µg, ATGA0518-100 µg, ATGA0518-250 µg, ATGA0518-500 µg, ATGA0518-1 mg, ATGA0518-10, ATGA0518-20, ATGA0518-50, ATGA0518-100, ATGA0518-250, ATGA0518-500, ATGA0518-1

References & Citations

Mario B. et al. (2007) J. Virology 81 (9) :4473-4481.; ; Ning S. et al. (2005) J. Virology 79:11671-11676.; ; Mario B. et al. (2007) J. Virology 81 (9) :4473-4481.

Other References

Moen SH, et al. Human Toll-like Receptor 8 (TLR8) Is an Important Sensor of Pyogenic Bacteria, and Is Attenuated by Cell Surface TLR Signaling. (Front Immunol. 2019) {https://pubmed.ncbi.nlm.nih.gov/31214180/}; ; Guo J, et al. A promising role of interferon regulatory factor 5 as an early warning biomarker for the development of human non-small cell lung cancer. (Lung Cancer. 2019) {https://pubmed.ncbi.nlm.nih.gov/31447002/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_116032.1

Uniprot Accession Number

Q13568

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: THP-1 cell lysate

FACS Description

Flow cytometry analysis of IRF5 in THP-1 cells. The cell was stained with ATGA0518 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained with ATGA0518 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of IRF-5 in Raw264.7 cells. The cell was stained with ATGA0518 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010457189584.jpg}