Human ActiCTA1 antibody

ACTA1 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Specifications

Product Name Alternative

ACTA, ACTA1, Actin, alpha 1, Alpha actin 1, Alpha skeletal muscle, Alpha skeletal muscle actin, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3, Skeletal muscle

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Actin (13-377aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2F5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Skeletal muscle, NEM3, NEM2, NEM1, MPFD, CFTDM, CFTD, ASMA, Alpha-actin-1, alpha1, Alpha skeletal muscle actin, Alpha skeletal muscle, Alpha actin 1, alpha 1, Actin, ACTA1, ACTA, ACTA 1, Alpha actin1, NEM 3, NEM 2, NEM 1, Actin, ATGA0517-10 µg, ATGA0517-20 µg, ATGA0517-50 µg, ATGA0517-100 µg, ATGA0517-250 µg, ATGA0517-500 µg, ATGA0517-1 mg, ATGA0517-010, ATGA0517-020, ATGA0517-050, ATGA0517-100, ATGA0517-250, ATGA0517-500, ATGA0517-01M

References & Citations

Moreno CAM , et al., (2017) Pediatr. Neurol. 75: 11-16. ; ; Loumaye A , et al., (2017) J Cachexia Sarcopenia Muscle 8 (5) : 768-777.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001091

Uniprot Accession Number

P68133

WB Description

The tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Actin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Lung Tissue lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: A549 cell lysate; ; Lane 4.: A431 cell lysate; ; Lane 5.: Raji cell lysate;

IF Description

ICC/IF analysis of Actin in HeLa cells. The cell was stained with ATGA0517 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .