Human NMNAT-1 antibody

NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults.

Product Specifications

Product Name Alternative

Nicotinamide nucleotide adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase, Leber's congenital amaurosis 9, LCA9, PNAT1, NMNAT

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NMNAT1 (19-609aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4G8

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PNAT1, PNAT 1, NMNAT1, NMNAT 1, NMNAT, NMN/NaMN adenylyltransferase1, NMN/NaMN adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase1, Nicotinamide nucleotide adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase, Leber's congenital amaurosis 9, LCA9, ATGA0516-10 µg, ATGA0516-20 µg, ATGA0516-50 µg, ATGA0516-100 µg, ATGA0516-250 µg, ATGA0516-500 µg, ATGA0516-1 mg, ATGA0516-010, ATGA0516-020, ATGA0516-050, ATGA0516-100, ATGA0516-250, ATGA0516-500, ATGA0516-01M

References & Citations

Emanuelli M. et al. (2001) J Biol Chem. 276 (1) :406-12.; ; Zhou T, ., et al. (2002) J Biol Chem. 277 (15) :13148-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_073624

Uniprot Accession Number

Q9HAN9

WB Description

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NMNAT-1 antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate (40ug) ; ; Lane 2.: NMNAT1 transfected 293T cell lysate (10 µg)