Human EpCAM/TROP-1 antibody

Epithelial cell adhesion molecule (EPCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EPCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EPCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EPCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EPCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

Product Specifications

Product Name Alternative

Epithelial cell adhesion molecules, CD326, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, DIAR5, EGP, GA733 2, HNPCC8

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human EpCAM/TROP-1 (24-265aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT36F10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

EpCAM, TROP1, ATGA0515-10 µg, ATGA0515-20 µg, ATGA0515-50 µg, ATGA0515-100 µg, ATGA0515-250 µg, ATGA0515-500 µg, ATGA0515-1 mg, ATGA0515-10, ATGA0515-20, ATGA0515-50, ATGA0515-100, ATGA0515-250, ATGA0515-500, ATGA0515-1

References & Citations

Litvinov. Sergey., et al. (1994) The Journal of Cell Biology 125 (2) : 437-46.; ; Maetzel D., et al. (2009) Nat Cell Biol. 11 (2) : 162-71.; ; Osta WA., et al. (2004) Cancer Res. 64 (16) : 5818-24.; ; Munz M., et al. (2004) Oncogene 23 (34) : 5748-58.; ; Armstrong A., et al. (2003) Cancer Biol Ther, 2 (4) : 320-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Tags & Cell Markers

NCBI Accession Number

NP_002345

Uniprot Accession Number

P16422

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EpCAM/TROP-1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; Lane 2.: PC3 cell lysate; ; Lane 3.: HeLa cell lysate

IF Description

ICC/IF analysis of EpCAM/TROP-1 in A431 cells. The cell was stained with ATGA0515 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .