Human PPAR gamma/NR1C3 antibody

PPAR gamma, also known as Peroxisome proliferator-activated receptor gamma, is member of the nuclear hormone receptor subfamily of transcription factors. It regulates transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. It is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. It also activators include prostanoids, fatty acids, thiazolidinediones and N- (2-benzoylphenyl) tyrosine analogues. PPARG may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake.

Product Specifications

Product Name Alternative

Peroxisome proliferator-activated receptor gamma, NR1C3, CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma, PPARG

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPARG (209-477aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4F5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PPAR g, ATGA0514-10 µg, ATGA0514-20 µg, ATGA0514-50 µg, ATGA0514-100 µg, ATGA0514-250 µg, ATGA0514-500 µg, ATGA0514-1 mg, ATGA0514-10, ATGA0514-20, ATGA0514-50, ATGA0514-100, ATGA0514-250, ATGA0514-500, ATGA0514-1

References & Citations

Berger J.. et al. (2002) Annu Rev Med . 53:409-35. ; ; Michalik L ., et al. (2006) Pharmacol Rev. 58 (4) :726-41.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_619725

Uniprot Accession Number

P37231

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPAR gamma antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate

FACS Description

Flow cytometry analysis of PPAR gamma in 293T cells. The cell was stained with ATGA0514 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of PPAR gamma in HeLa cells. The cell was stained with ATGA0514 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .