Human Iduronate 2-Sulfatase/IDS antibody

IDS, as known asiduronate 2-sulfatase isoform, isa member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O-sulfate and N-salfate esters from a varity of substrates. This protein is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate. It hydrolyzes the 2-sulfate group of the IDS units of the GAG.

Product Specifications

Product Name Alternative

Iduronate 2-sulfatase isoform, IDS, MPS2, SIDS

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IDS (26-550aa) purified from insect cell

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT6H4

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Iduronate2-Sulfatase, IDS, ATGA0513-10 µg, ATGA0513-20 µg, ATGA0513-50 µg, ATGA0513-100 µg, ATGA0513-250 µg, ATGA0513-500 µg, ATGA0513-1 mg, ATGA0513-10, ATGA0513-20, ATGA0513-50, ATGA0513-100, ATGA0513-250, ATGA0513-500, ATGA0513-1

References & Citations

Torres LC., et al, (2014) Clin. Immunol. 154:100-104.; ; Chistiakov DA., et al, (2014) J.Genet.Genomics. 41:197-203.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Other

NCBI Accession Number

NP_000193

Uniprot Accession Number

P22304

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IDS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human IDS

FACS Description

Flow cytometry analysis of IDS in HeLa cells. The cell was stained with ATGA0513 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .