Human Erythropoietin/EPO antibody

EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types.

Product Specifications

Product Name Alternative

EPO, EP, MVCD2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Erythropoietin (28-193aa) purified from insect cell

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1C12

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Erythropoietin, ATGA0512-10 µg, ATGA0512-20 µg, ATGA0512-50 µg, ATGA0512-100 µg, ATGA0512-250 µg, ATGA0512-500 µg, ATGA0512-1 mg, ATGA0512-10, ATGA0512-20, ATGA0512-50, ATGA0512-100, ATGA0512-250, ATGA0512-500, ATGA0512-1

References & Citations

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263.; ; Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Angiogenesis

NCBI Accession Number

NP_000790

Uniprot Accession Number

P01588

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPO antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: EPO transfected 293T cell lysate