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Human Otubain-2/OTUB2 antibody

OTUB2 (Ubiquitin thioesterase OTUB2) belongs to the peptidase C65 family. OTUB2 functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.

Product Specifications

Product Name Alternative

Ubiquitin thioesterase OTuB2, OTB2, OTu2, TimntanOTUB2 aUgitin thioesterase 2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human OTUB2 (1-234aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1F8

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

OTUB2, ATGA0511-10 µg, ATGA0511-20 µg, ATGA0511-50 µg, ATGA0511-100 µg, ATGA0511-250 µg, ATGA0511-500 µg, ATGA0511-1 mg, ATGA0511-10, ATGA0511-20, ATGA0511-50, ATGA0511-100, ATGA0511-250, ATGA0511-500, ATGA0511-1

References & Citations

Balakirev MY, et al. (2003) EMBO Rep 4 (5) : 517-22.; ; Edelmann M.J., et al. (2009) Biochem. J. 418:379-390.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_075601

Uniprot Accession Number

Q96DC9

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OTUB2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: OTUB2 transfected 293T cell lysate

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