Human Histone Deacetylase 2/HDAC2 antibody

HDAC2 belongs to the histone deacetylase family that act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones. It forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. It also plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Product Specifications

Product Name Alternative

Histone deacetylase 2, HD2, RPD3, YAF1, KDAC2, Protein deacylase HDAC2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human HDAC2 (1-488aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT3B1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Histone Deacetylase2, HDAC2, ATGA0510-10 µg, ATGA0510-20 µg, ATGA0510-50 µg, ATGA0510-100 µg, ATGA0510-250 µg, ATGA0510-500 µg, ATGA0510-1 mg, ATGA0510-10, ATGA0510-20, ATGA0510-50, ATGA0510-100, ATGA0510-250, ATGA0510-500, ATGA0510-1

References & Citations

Betz R. et al. (1998) Genomics. 52:245-6.; ; Taunton J. et al. (1996) Science 272:408-11.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001518

Uniprot Accession Number

Q92769

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HDAC2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human HDAC2

FACS Description

Flow cytometry analysis of HDAC2 in A549 cells. The cell was stained with ATGA0510 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .

IF Description

ICC/IF analysis of HDAC2 in HeLa cells. The cell was stained with ATGA0510 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .