Human TRAILR3/TNFRSF10C antibody

TNFRSF10C also known as tumor necrosis factor receptor superfamily member 10C is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. This receptor is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis.

Product Specifications

Product Name Alternative

Tumor necrosis factor receptor superfamily member 10C, Antagonist decoy receptor for TRAIL/Apo-2L, Decoy TRAIL receptor without death domain, Decoy receptor 1, DcR1, Lymphocyte inhibitor of TRAIL, TNF-related apoptosis-inducing ligand receptor 3, TRAIL receptor 3, TRAIL-R3, CD263, LIT, TRAILR3, TRID, TRAIL receptor without an intracellular domain

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TNFRSF10C (26-236aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1E12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TRAIL R3, TNFRSF10C, ATGA0509-10 µg, ATGA0509-20 µg, ATGA0509-50 µg, ATGA0509-100 µg, ATGA0509-250 µg, ATGA0509-500 µg, ATGA0509-1 mg, ATGA0509-10, ATGA0509-20, ATGA0509-50, ATGA0509-100, ATGA0509-250, ATGA0509-500, ATGA0509-1

References & Citations

Venza M., et al. (2013) Biochem. Biophys. Res. Commun. 441 (4), 743-750.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_003832

Uniprot Accession Number

O14798

WB Description

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TNFRFS10C antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Mouse Liver tissue lysate

IF Description

ICC/IF analysis of TNFRSF10C in HeLa cells. The cell was stained with ATGA0509 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .