Human Ornithine Decarboxylase/ODC1 antibody

Ornithine decarboxylase, also known as ODC1, is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. ODC1 plays an important role in diverse biological processes, including cell growth, differentiation, transformation and apoptosis. Overexpression of the ODC1 gene plays important roles in cell proliferation and the development of cancer.

Product Specifications

Product Name Alternative

Ornithine decarboxylase, ODC

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ODC1 (1-461aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT9B3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Ornithine Decarboxylase, ATGA0507-10 µg, ATGA0507-20 µg, ATGA0507-50 µg, ATGA0507-100 µg, ATGA0507-250 µg, ATGA0507-500 µg, ATGA0507-1 mg, ATGA0507-10, ATGA0507-20, ATGA0507-50, ATGA0507-100, ATGA0507-250, ATGA0507-500, ATGA0507-1

References & Citations

Moshier J A., et al. (1992) Genomics. 1:77-86.; ; Yang Feng T L., et al. (1996) Genomics. 36:449-458.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002530

Uniprot Accession Number

P11926

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ODC1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate ; ; Lane 2.: ODC1 transfected 293T cell lysate

IF Description

ICC/IF analysis of ODC1 in HeLa cells. The cell was stained with ATGA0507 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .