Human Calcineurin A/PPP3CA antibody

PPP3CA, also known as Calcineurin A, is a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. It activates the T cells of the immune system and can be blocked by drugs. PPP3CA activates NFATc, a transcription factor by dephosphorylating it. The activated NFATc is then translocated into the nucleus, where it upregulates the expression of interleukin 2.

Product Specifications

Product Name Alternative

Protein phosphatase 3 catalytic subunit alpha, CAM-PRP catalytic subunit, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, CNA alpha, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CALNA, CALN, CNA, CNA1, PPP2B

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPP3CA (1-511aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1E11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PPP3CA, ATGA0505-10 µg, ATGA0505-20 µg, ATGA0505-50 µg, ATGA0505-100 µg, ATGA0505-250 µg, ATGA0505-500 µg, ATGA0505-1 mg, ATGA0505-10, ATGA0505-20, ATGA0505-50, ATGA0505-100, ATGA0505-250, ATGA0505-500, ATGA0505-1

References & Citations

Ostenfeld MS., et al. (2010) Oncogene. 29 (7) :1073-84.; ; Rodriguez A., et al. (2009) Mol Cell. 33 (5) :616-26.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_001124163

Uniprot Accession Number

Q08209

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP3CA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: 293T cell lysate ; ; Lane 3.: LNCaP cell lysate

IF Description

ICC/IF analysis of PPP3CA in HeLa cells. The cell was stained with ATGA0505 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .