DYKDDDDK Epitope Tag antibody

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins with in living cells. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

Product Specifications

Product Name Alternative

FLAG tag

Host

Mouse

Immunogen

Synthetic peptide (DYKDDDDKC) -KLH

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3G6

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

DYKDDDDK Epitope Tag, ATGA0504-10 µg, ATGA0504-20 µg, ATGA0504-50 µg, ATGA0504-100 µg, ATGA0504-250 µg, ATGA0504-500 µg, ATGA0504-1 mg, ATGA0504-10, ATGA0504-20, ATGA0504-50, ATGA0504-100, ATGA0504-250, ATGA0504-500, ATGA0504-1

References & Citations

Dave JM, et al (2016), J Cell Sci 129:743-56.; ; Guo H, et al (2016), Genome Biol 17:24.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Other

WB Description

The recombinant protein (200ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Flag tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human 6XHis-SNCA-3xFlag The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Flag tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: ODC1-Flag tag Transfected 293T cell lysate {ATGA0504-WB2.jpg} The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Flag tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HDAC2-Flag tag Transfected 293T cell lysate {ATGA0504-WB3.jpg}