Human MP1/LAMTOR3 antibody

MAPKSP1 (Mitogen-activated protein kinase scaffold protein 1) is a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase scaffold protein 1, MAP2K1IP1, MAPBP, MP1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MAPKSP1 (1-124aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1F10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MP1, ATGA0503-10 µg, ATGA0503-20 µg, ATGA0503-50 µg, ATGA0503-100 µg, ATGA0503-250 µg, ATGA0503-500 µg, ATGA0503-1 mg, ATGA0503-10, ATGA0503-20, ATGA0503-50, ATGA0503-100, ATGA0503-250, ATGA0503-500, ATGA0503-1

References & Citations

Wunderlich, et al. (2001) J. Cell Biol. (United States) 152 (4) : 765-76. ; ; Schaeffer HJ, et al. (1998) Science 281 (5383) : 1668-71.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_068805

Uniprot Accession Number

Q9UHA4

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: MAPKSP1 transfected 293T cell lysate

IF Description

ICC/IF analysis of MP1 in HaLa cells. The cell was stained with ATGA0503 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .