Human XPA antibody

XPA, also known as DNA repair protein complementing XP-A cells, belong to the XPA family. This protein is involved in DNA excision repair. It Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A), which is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities.

Product Specifications

Product Name Alternative

DNA repair protein complementing XP-A cells, XP1, XPAC

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human XPA (1-273aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT71H3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

XPA, ATGA0502-10 µg, ATGA0502-20 µg, ATGA0502-50 µg, ATGA0502-100 µg, ATGA0502-250 µg, ATGA0502-500 µg, ATGA0502-1 mg, ATGA0502-10, ATGA0502-20, ATGA0502-50, ATGA0502-100, ATGA0502-250, ATGA0502-500, ATGA0502-1

References & Citations

Pan Y.R., et al. (2009) Cell Cycle 8:655-664.; ; James, et al. (2005) Andrews Diseases of the Skin: Clinical Dermatology. (10th ed.) . Saunders.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000371

Uniprot Accession Number

P23025

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: SW480 cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: MCF7 cell lysate ; ; Lane 4.: LNCaP cell lysate; ; Lane 5.: Raji cell lysate

IF Description

ICC/IF analysis of XPA in HaLa cells. The cell was stained with ATGA0502 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .