Human NMT1 antibody

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO. Recombinant human NMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glycylpeptide N-tetradecanoyltransferase 1, Glycylpeptide N-tetradecanoyltransferase 1, NMT, Myristoyl-CoA:protein N-myristoyltransferase 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NMT1 (1-496aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2C8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NMT1, ATGA0501-10 µg, ATGA0501-20 µg, ATGA0501-50 µg, ATGA0501-100 µg, ATGA0501-250 µg, ATGA0501-500 µg, ATGA0501-1 mg, ATGA0501-10, ATGA0501-20, ATGA0501-50, ATGA0501-100, ATGA0501-250, ATGA0501-500, ATGA0501-1

References & Citations

Glover C.J. et al. (1997) J. Biol. Chem. 272:28680-28689.; ; Van Damme P. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_066565

Uniprot Accession Number

P30419

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NMT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate ; ; Lane 2.: 293T cell lysate

IF Description

ICC/IF analysis of NMT1 in HeLa cells. The cell was stained with ATGA0501 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .