HA Tag antibody

HA tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a HA-tag allows one to follow the protein with an antibody against the HA epitope.

Product Specifications

Product Name Alternative

Influenza Hemagglutinin epitope

Host

Mouse

Antigen Species

Human

Immunogen

Synthetic peptide (YPYDVPDYA) -KLH

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT5E7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HA Tag, ATGA0499-10 µg, ATGA0499-20 µg, ATGA0499-50 µg, ATGA0499-100 µg, ATGA0499-250 µg, ATGA0499-500 µg, ATGA0499-1 mg, ATGA0499-10, ATGA0499-20, ATGA0499-50, ATGA0499-100, ATGA0499-250, ATGA0499-500, ATGA0499-1

References & Citations

Field. J, et al (1988) Mol. Cell. Biol. 8: 2159-2165.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Fusion Tag & Detection

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: PDPN-HA tag Transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant HA tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: EPO-HA tag Transfected 293T cell lysate {ATGA0499-WB2.jpg} The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human FABP2-HA tag {ATGA0499-WB3.jpg} The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human MIF-HA tag` {ATGA0499-WB4.jpg}