Human Glutathione S-transferase pi 1/GSTP1 antibody

GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur) . The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.

Product Specifications

Product Name Alternative

Glutathione S-transferase P, DFN7, FAEES3, GST3, PI, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III, Glutathione S Transferase Pi, GSTP1, Glutathione S-Transferase pi 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GSTP1 (1-210aa) purified from E.coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT12C10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Glutathione S-Transferase pi1, GSTP1, ATGA0498-10 µg, ATGA0498-20 µg, ATGA0498-50 µg, ATGA0498-100 µg, ATGA0498-250 µg, ATGA0498-500 µg, ATGA0498-1 mg, ATGA0498-10, ATGA0498-20, ATGA0498-50, ATGA0498-100, ATGA0498-250, ATGA0498-500, ATGA0498-1

References & Citations

Lee KA, et al. (2001) Blood. 98 (12) : 3483-5.; ; Hayes JD, et al. (1995) Crit Rev BiochemMol Biol. 30 (6) : 445-600.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_000843

Uniprot Accession Number

P09211

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: K562 cell lysate ; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: 293T cell lysate; ; Lane 5.: A549 cell lysate

FACS Description

Flow cytometry analysis of GSTP1 in K562 cell line. The cell was stained with ATGA0498 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of GSTP1 in A549 cells. The cell was stained with ATGA0498 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .