Human CD34 antibody

CD34, also known as hematopoietic progenitor cell antigen CD34 isoform b, is a member of a family of single-pass transmembrane sialomucin proteins. It is adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. This protein could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.

Product Specifications

Product Name Alternative

Hematopoietic progenitor cell antigen CD34 isoform b, CD34

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CD34 (32-290aa) purified from Baculovirus.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3B6

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CD34, Hematopoietic progenitor cell antigen CD34 isoform b, CD34, ATGA0497-10 µg, ATGA0497-20 µg, ATGA0497-50 µg, ATGA0497-100 µg, ATGA0497-250 µg, ATGA0497-500 µg, ATGA0497-1 mg, ATGA0497-10, ATGA0497-20, ATGA0497-50, ATGA0497-100, ATGA0497-250, ATGA0497-500, ATGA0497-1

References & Citations

Nielsen JS., et al. (2009) Microcirculation. 16: 487-496.; ; Mastrandrea F., et al. (2008) Eur Ann Allergy Clin Immunol. 40: 90-103.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_001764

Uniprot Accession Number

P28906

WB Description

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD34 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: 293T cell lysate

FACS Description

Flow cytometry analysis of CD34 in KG1 cells. The cell was stained with ATGA0497 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .