What is the catalog number for Human PDHX antibody?

The catalog number for Human PDHX antibody is ATGA0494-050.

What sizes are available for Human PDHX antibody?

Human PDHX antibody is available in the following size(s): 50 µL.

Is Human PDHX antibody in stock?

Human PDHX antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human PDHX antibody

The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP) . This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex.

Product Specifications

Product Name Alternative

Pyruvate dehydrogenase protein X component mitochondrial, Pyruvate dehydrogenase protein X component, mitochondrial, DLDBP, E3BP, OPDX, PDX1, proX

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PDHX (54-501aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1E11

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PDHX, Pyruvate dehydrogenase protein X component mitochondrial, Pyruvate dehydrogenase protein X component, mitochondrial, DLDBP, E3BP, OPDX, PDX1, proX, ATGA0494-10 µg, ATGA0494-20 µg, ATGA0494-50 µg, ATGA0494-100 µg, ATGA0494-250 µg, ATGA0494-500 µg, ATGA0494-1 mg, ATGA0494-10, ATGA0494-20, ATGA0494-50, ATGA0494-100, ATGA0494-250, ATGA0494-500, ATGA0494-1

References & Citations

Aral B., et al. (1997) Am. J. Hum. Genet. 61: 1318-1326.; ; Hiromasa Y., et al. (2004) J. Biol. Chem. 279: 6921-6933.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003468

Uniprot Accession Number

O00330

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDHX antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A431 cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: LnCap cell lysate