Human ATG3 antibody

Ubiquitin-like-conjugating enzyme ATG3 isoform 1, also known as ATG3, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate, a reaction that is essential for autophagy.

Product Specifications

Product Name Alternative

Ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ATG3 (1-314aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT15F6

Conjugation

Unconjugated

Applications

ELISA, WB, FACS, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ATG3, ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96, ATGA0493-10 µg, ATGA0493-20 µg, ATGA0493-50 µg, ATGA0493-100 µg, ATGA0493-250 µg, ATGA0493-500 µg, ATGA0493-1 mg, ATGA0493-10, ATGA0493-20, ATGA0493-50, ATGA0493-100, ATGA0493-250, ATGA0493-500, ATGA0493-1

References & Citations

Tanida I., et al. (2002) J Biol Chem. 277: 13739-13744. ; ; Yamada Y., et al. (2007) J Biol Chem. 282: 8036-8043.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_071933

Uniprot Accession Number

Q9NT62

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATG3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: ATG3 transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATG3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: K562 cell lysate ; ; Lane 2.: Raji cell lysate ; ; Lane 3.: Jurkat cell lysate {ATGA0493-WB2.jpg}

FACS Description

Flow cytometry analysis of ATG3 in HeLa cells. The cell was stained with ATGA0493 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of ATG3 in HeLa cells. The cell was stained with ATGA0493 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0493-IF1b.jpg}