Human Sulfotransferase 2A1/SULT2A1 antibody

SULT2A1, also known dehydroepiandrosterone sulphotransferase, belongs to the sulfotransferase family. This protein is mainly expressed in liver and adrenal tissues, and to a lesser extent in kidney. It catalyzes the 3'-phosphoadenosine 5'-phosphosulfate-dependent sulfation of a wide variety of steroids in human liver and adrenal tissues, and is also responsible for most of the sulfation of bile acids in human liver.

Product Specifications

Product Name Alternative

Sulfotransferase family 2A member 1, ST2A1, Cytosolic sulfotransferase family 2A, Bile salt sulfotransferase, Dehydroepiandrosterone sulfotransferase, DHEA-ST, DHEA-ST8, Hydroxysteroid Sulfotransferase, HST, ST2, SULT2A3, STD

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SULT2A1 (1-285aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT13E10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cytosolic Sulfotransferase2A1, SULT2A1, Bile salt sulfotransferase, DHEA-ST, DHEAS, HST, hSTa, ST2, ST2A1, ST2A3, ATGA0491-10 µg, ATGA0491-20 µg, ATGA0491-50 µg, ATGA0491-100 µg, ATGA0491-250 µg, ATGA0491-500 µg, ATGA0491-1 mg, ATGA0491-10, ATGA0491-20, ATGA0491-50, ATGA0491-100, ATGA0491-250, ATGA0491-500, ATGA0491-1

References & Citations

Otterness D.M. et al. (1992) Mol. Pharmacol. 41: 865-872. ; ; Comer K.A. et al. (1993) Biochem. J. 289: 233-240.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003158

Uniprot Accession Number

Q06520

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SULT2A1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SULT2A1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: SULT2A1 transfected 293T cell lysate ; ; Lane 2.: 293T cell lysate {ATGA0491-WB2.jpg}

IF Description

ICC/IF analysis of SULT2A1 in Hep3B The cell was stained with ATGA0491 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .