Human FIS1 antibody

TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. This protein can induce cytochrome C release from the mitochondrion to the cytosol, ultimately leading to apoptosis. It is also involved in peroxisomal growth and division. The C terminus is required for mitochondrial localisation, while the N teminus is necessary for mitochondrial fission.

Product Specifications

Product Name Alternative

Mitochondrial fission 1 protein, FIS1 homolog, hFis1, Tetratricopeptide repeat protein 11, TPR repeat protein 11, TTC11, CGI-135 protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TTC11 (1-122aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3B7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CGI 135, CGI135, FIS 1, FIS1, Fis1 homolog, Fission 1 (mitochondrial outer membrane) homolog, Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae), Fission 1 (mitochondrial outer membrane) homolog (yeast) Fission 1 homolog, H NH0132A01.6, hFis 1, hFis1, homolog of, Mitochondrial fission 1 protein, S. cerevisiae, "S, cerevisiae", Tetratricopeptide repeat domain 11, Tetratricopeptide repeat domain 11 2010003O14Rik, Tetratricopeptide repeat protein 11, TPR repeat protein 11, TTC 11, ATGA0490-10 µg, ATGA0490-20 µg, ATGA0490-50 µg, ATGA0490-100 µg, ATGA0490-250 µg, ATGA0490-500 µg, ATGA0490-1 mg, ATGA0490-010, ATGA0490-020, ATGA0490-050, ATGA0490-100, ATGA0490-250, ATGA0490-500, ATGA0490-01M

References & Citations

Martinou JC., et al. (2003) J Biol Chem. 278 (38) : 36373-9. ; ; Yoon Y., et al. (2008) Exp Cell Res. 314 (19) : 3494-507.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_057152

Uniprot Accession Number

Q9Y3D6

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TTC11 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: MCF7 cell lysate The cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TTC11 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: TTC11 transfected 293T cell lysate {ATGA0490-WB2.jpg}

IF Description

ICC/IF analysis of TTC11 in HaLa cells. The cell was stained with ATGA0490 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .