Human MCM7 antibody

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB.

Product Specifications

Product Name Alternative

DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MCM7 (1-414aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1G8

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MCM7, DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, ATGA0489-10 µg, ATGA0489-20 µg, ATGA0489-50 µg, ATGA0489-100 µg, ATGA0489-250 µg, ATGA0489-500 µg, ATGA0489-1 mg, ATGA0489-10, ATGA0489-20, ATGA0489-50, ATGA0489-100, ATGA0489-250, ATGA0489-500, ATGA0489-1

References & Citations

Zhou, Y.M., et al. (2012) Liver Int. 32 (10), 1505-1509. ; ; Liu, Y.Z., et al. (2012) Lung Cancer 77 (1), 176-182.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_877577

Uniprot Accession Number

P33993

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MCM7 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: HepG2 cell lysate

FACS Description

Flow cytometry analysis of MCM7 in HeLa cells. The cell was stained with ATGA0489 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .