Human alpha-FetoproteiFP antibody

AFP, also known as alpha-fetoprotein, is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. This protein binds to copper, nickel, fatty acids and bilirubin. The main function of this is to prevent the virilization of female fetuses. As it does not bind estrogen, its function in humans is less clear.

Product Specifications

Product Name Alternative

Alpha-fetoprotein, AFPD, FETA, HPAFP

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AFP (19-609aa) purified from insect cell.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT73E10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

A-Fetoprotein, AFP, ATGA0488-10 µg, ATGA0488-20 µg, ATGA0488-50 µg, ATGA0488-100 µg, ATGA0488-250 µg, ATGA0488-500 µg, ATGA0488-1 mg, ATGA0488-10, ATGA0488-20, ATGA0488-50, ATGA0488-100, ATGA0488-250, ATGA0488-500, ATGA0488-1

References & Citations

Tomasi TB Jr., (1997) Annu Rev Med. 28: 453-465. ; ; Elbrecht A, et al. (1992) Science. 255: 467-470.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001125

Uniprot Accession Number

P02771

WB Description

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AFP antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate ; ; Lane 2.: Mouse liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AFP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: Raw264.7 cell lysate {ATGA0488-WB2.jpg}

FACS Description

Flow cytometry analysis of AFP in HeLa cells. The cell was stained with ATGA0488 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of AFP in HaLe cells. The cell was stained with ATGA0488 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .