Human CD94/KLRD1 antibody

CD94 (Cluster of Differentiation 94), also known as killer cell lectin-like receptor subfamily D, member 1 (KLRD1) is a human gene. The protein encoded by CD94 gene is a lectin, cluster of differentiation and a receptor that is involved in cell signaling and is expressed on the surface of natural killer cells in the innate immune system. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA) -E on target cells.

Product Specifications

Product Name Alternative

Killer cell lectin like receptor D1, Natural killer cells antigen CD94, KP43, Killer cell lectin-like receptor subfamily D member 1, NK cell receptor

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KLRD1 (32-179aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT13E3

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CD94, ATGA0487-10 µg, ATGA0487-20 µg, ATGA0487-50 µg, ATGA0487-100 µg, ATGA0487-250 µg, ATGA0487-500 µg, ATGA0487-1 mg, ATGA0487-10, ATGA0487-20, ATGA0487-50, ATGA0487-100, ATGA0487-250, ATGA0487-500, ATGA0487-1

References & Citations

Cheent KS., et al. (2013) pnas. 110 (42) : 16981-16986.; ; Shum BP., et al. (2008) Nat Immunol. 168 (1) : 240-252.; ; Fang M., et al. (2011) Immunity 34 (4) : 579-589.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_002253

Uniprot Accession Number

Q13241

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLRD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: K562 cell lysate ; ; Lane 2.: MCF7 cell lysate ; ; Lane 3.: A549 cell lysate The NK-92 cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLRD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0487-WB2.jpg}

FACS Description

Flow cytometry analysis of KLRD1 in PBMC cells. The cell was stained with ATGA0487 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .