Human FHL2 antibody

FHL2 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Specifications

Product Name Alternative

Four and a half LIM domains 2, AAG11, DRAL, FHL-2, SLIM-3, SLIM3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FHL2 (1-279aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT21D11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

FHL2, Four and a half LIM domains 2, AAG11, DRAL, FHL-2, SLIM-3, SLIM3, ATGA0486-10 µg, ATGA0486-20 µg, ATGA0486-50 µg, ATGA0486-100 µg, ATGA0486-250 µg, ATGA0486-500 µg, ATGA0486-1 mg, ATGA0486-10, ATGA0486-20, ATGA0486-50, ATGA0486-100, ATGA0486-250, ATGA0486-500, ATGA0486-1

References & Citations

Verset, L., et al. (2013) Br. J. Cancer. 109 (1), 114-120. ; ; Han, W., et al. (2013) J. Clin. Invest. 123 (5), 2103-2118.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001034581

Uniprot Accession Number

Q14192

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FHL2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: A549 cell lysate ; ; Lane 3.: MCF7 cell lysate ; ; Lane 4.: SK-OV-3 cell lysate ; ; Lane 5.: HT1080 cell lysate

IF Description

ICC/IF analysis of FHL2 in HeLa cells. The cell was stained with ATGA0486 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0486-IF1b.jpg}