Human Integrin beta 1/CD29 antibody

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Product Specifications

Product Name Alternative

Integrin beta 1, Integrin beta 1, CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, Fibronectin receptor subunit beta, Glycoprotein Iia, GPIIA, VLA-4 subunit bet

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ITGB1 (21-461aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT47E2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

VLA-BETA, VLAB, VLA-4 subunit beta, NRK2, Nicotinamide riboside kinase 2, MSK12, MIBP, MDF2, ITGB1, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta-1 isoform 1A, Integrin beta 1, Integrin b1, Il-6, IL6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0485-10 µg, ATGA0485-20 µg, ATGA0485-50 µg, ATGA0485-100 µg, ATGA0485-250 µg, ATGA0485-500 µg, ATGA0485-1 mg, ATGA0485-010, ATGA0485-020, ATGA0485-050, ATGA0485-100, ATGA0485-250, ATGA0485-500, ATGA0485-01M

References & Citations

Pellinen T., et al. (2008) Dev. Cell. 15: 371-385.; ; Chuang N.N., et al. (2007) Biochem. Soc. Trans. 35:1292-1294.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_002202

Uniprot Accession Number

P05556

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ITGB1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A549 cell lysate ; ; Lane 2.: PC3 cell lysate ; ; Lane 3.: HT1080 cell lysate

IF Description

ICC/IF analysis of ITGB1 in HeLa cells. The cell was stained with ATGA0485 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0485-IF1b.jpg}

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