Human Calnexin antibody

Calnexin, also known as IP90, p88 and p90, is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, endoplasmic reticulum (ER) -associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Product Specifications

Product Name Alternative

CNX, IP90, P90, CANX, FLJ26570, Histocompatibility complex class I antigen binding protein p88

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Calnexin (21-481aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT18B9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Calnexin, ATGA0483-10 µg, ATGA0483-20 µg, ATGA0483-50 µg, ATGA0483-100 µg, ATGA0483-250 µg, ATGA0483-500 µg, ATGA0483-1 mg, ATGA0483-10, ATGA0483-20, ATGA0483-50, ATGA0483-100, ATGA0483-250, ATGA0483-500, ATGA0483-1

References & Citations

Wang B., et al. (2009) Immunology. 128 (1) : 43-57.; ; Millar DJ., et al. (2009) Proteomics. 9 (9) : 2355-72.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001019820

Uniprot Accession Number

P27824

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calnexin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: MCF7 cell lysate ; ; Lane 3.: A431 cell lysate ; ; Lane 4.: A549 cell lysate; ; Lane 5.: 293T cell lysate; ; Lane 6.: HepG2 cell lysate; ; Lane 7.: Jurkat cell lysate

FACS Description

Flow cytometry analysis of Calnexin in HeLa cells. The cell was stained with ATGA0483 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .Flow cytometry analysis of Calnexin in A549 cells. The cell was stained with ATGA0483 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .{ATGA0483-FC2.jpg}

IF Description

ICC/IF analysis of Calnexin in HeLa cells. The cell was stained with ATGA0483 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .