Human MCM7 antibody

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB.

Product Specifications

Product Name Alternative

DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MCM7 (1-414aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT118H7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MCM7, DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, ATGA0481-10 µg, ATGA0481-20 µg, ATGA0481-50 µg, ATGA0481-100 µg, ATGA0481-250 µg, ATGA0481-500 µg, ATGA0481-1 mg, ATGA0481-10, ATGA0481-20, ATGA0481-50, ATGA0481-100, ATGA0481-250, ATGA0481-500, ATGA0481-1

References & Citations

Zhou Y.M., et al. (2012) Liver Int. 32 (10) : 1505-1509.; ; Liu, Y.Liu Y.Z., et al. (2012) Lung Cancer. 77 (1) : 176-182.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_877577

Uniprot Accession Number

P33993

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MCM7 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: K562 cell lysate

IF Description

ICC/IF analysis of MCM7 in MCF7 cells. The cell was stained with ATGA0481 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0481-IF1b.jpg}