Human Akt1 antibody

AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Product Specifications

Product Name Alternative

Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AKT1 (1-480aa) purified from insect cell.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT6D5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Akt1, RAC-alpha serine/threonine-protein kinase, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, ATGA0480-10 µg, ATGA0480-20 µg, ATGA0480-50 µg, ATGA0480-100 µg, ATGA0480-250 µg, ATGA0480-500 µg, ATGA0480-1 mg, ATGA0480-10, ATGA0480-20, ATGA0480-50, ATGA0480-100, ATGA0480-250, ATGA0480-500, ATGA0480-1

References & Citations

Lindhurst MJ, et al (2011) N Engl J Med. 365 (7) : 611-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001014432

Uniprot Accession Number

P31749

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: NIH3T3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human AKT1 ; ; Lane 2.: Recombinant Human AKT2 ; ; Lane 3.: Recombinant Human AKT3 {ATGA0480-WB2.jpg}

FACS Description

Flow cytometry analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0480-IF1b.jpg}