Human TUBG1 antibody

TUBG1 is a member of the tubulin superfamily. This protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. It mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle.

Product Specifications

Product Name Alternative

Tubulin gamma-1 chain, GCP-1, TuBG, TuBGCP1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TUBG1 (1-451aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT9D6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TUBG1, Tubulin gamma-1 chain, GCP-1, TuBG, TuBGCP1, ATGA0479-10 µg, ATGA0479-20 µg, ATGA0479-50 µg, ATGA0479-100 µg, ATGA0479-250 µg, ATGA0479-500 µg, ATGA0479-1 mg, ATGA0479-10, ATGA0479-20, ATGA0479-50, ATGA0479-100, ATGA0479-250, ATGA0479-500, ATGA0479-1

References & Citations

Zheng Y., et al. (1991) Cell. 65 (5) : 817-23.; ; Wise DO., et al. (2000) Genomics. 67 (2) : 164-70.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001061

Uniprot Accession Number

P23258

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TUBG1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: A549 cell lysate ; ; Lane 4.: K562 cell lysate

IF Description

ICC/IF analysis of TUBG1 in HeLa cells. The cell was stained with ATGA0479 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0479-IF1b.jpg}