Human Angiopoietin-like Protein 3/ANGPTL3 antibody

ANGPTL3 also known as Angiopoietin-related protein 3 is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN) -like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis.

Product Specifications

Product Name Alternative

ANG-5, Angiopoietin 5, Angiopoietin-related protein 3, ANGPT5, ANGPTL3, ANL3, FHBL2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ANGPT5 (243-460aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT23D8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Angiopoietin-like Protein3, ANGPTL3, Angiopoietin-related protein 3, ANG-5, ANGPT5, ANL3, FHBL2, ATGA0478-10 µg, ATGA0478-20 µg, ATGA0478-50 µg, ATGA0478-100 µg, ATGA0478-250 µg, ATGA0478-500 µg, ATGA0478-1 mg, ATGA0478-10, ATGA0478-20, ATGA0478-50, ATGA0478-100, ATGA0478-250, ATGA0478-500, ATGA0478-1

References & Citations

Arca M., et al. (2013) Curr. Opin. Lipidol. 24 (4) : 313-20.; ; Quagliarini F Q., et al. (2012) Proc Natl Acad Sci. 109 (48) : 19751-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_055310

Uniprot Accession Number

Q9Y5C1

WB Description

The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ANGPT5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Mouse kidney tissue lysate

IF Description

ICC/IF analysis of ANGPT5 in HeLa cells. The cell was stained with ATGA0478 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .