Human DKK3 antibody

Dickkopf-related protein 3, also known as DKK3, is a member of the DKK protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. DKK3 is a 350 amino acid secreted glycoprotein that is composed of an N-terminal signal peptide and two conserved cysteine-rich domains, which are separated by a 12 amino acid linker region. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Product Specifications

Product Name Alternative

Dickkopf WNT signaling pathway inhibitor 3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, REIC, RIG, CRRL

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human DKK3 (22-350aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT103B5

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cardiomyocyte regeneration-related lncRNA, CRRL, Dickkopf, Dickkopf 3 homolog, Dickkopf WNT signaling pathway inhibitor 3, Dickkopf-3, Dickkopf-related protein 3, Dickkopf-related protein 3 precursor, DKK3, Dkk-3, Regulated in glioma, REIC, RIG, Xenopus laevis, Xenopus laevis homolog 3, ATGA0476-10 µg, ATGA0476-20 µg, ATGA0476-50 µg, ATGA0476-100 µg, ATGA0476-250 µg, ATGA0476-500 µg, ATGA0476-1 mg, ATGA0476-010, ATGA0476-020, ATGA0476-050, ATGA0476-100, ATGA0476-250, ATGA0476-500, ATGA0476-01M

References & Citations

Cadigan K M., et al. (1997) Genes Dev. 11: 3286-3305.; ; Gilnka A., et al. (1998) Nature. 391: 357-362.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001018067

Uniprot Accession Number

Q9UBP4

WB Description

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DKK3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: A549 cell lysate; ; Lane 5.: Mouse brain tissue lysate; ; Lane 6.: Mouse kidney tissue lysate