Human Glutamine Synthetase antibody

Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency.

Product Specifications

Product Name Alternative

Glutamine synthase, Glutamate-ammonia ligase, GLNS, Glutamate-ammonia ligase, GS, Palmitoyltransferase GLUL

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GLUL (1-373aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT8D7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GLUL, Glutamine synthetase , GLNS, GS, PIG43, PIG59, cell proliferation-inducing protein 59, GLNA, GLNA_HUMAN, GLNS, GLULGlutamate ammonia ligase, Glutamate decarboxylaseGlutamate--ammonia ligase, glutamine synthaseGlutamine synthetase, GS, PIG 43, PIG 59, PIG43PIG59, Proliferation inducing protein 43, ATGA0474-10 µg, ATGA0474-20 µg, ATGA0474-50 µg, ATGA0474-100 µg, ATGA0474-250 µg, ATGA0474-500 µg, ATGA0474-1 mg, ATGA0474-10, ATGA0474-20, ATGA0474-50, ATGA0474-100, ATGA0474-250, ATGA0474-500, ATGA0474-1

References & Citations

Vermeulen T., et al, (2008) Arch Biochem Biophys. 478: 96-102. ; ; Liaw SH., et al. (1995) Protein Sci. 4 (11) : 2358-65.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_001028216

Uniprot Accession Number

P15104

WB Description

The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GLUL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Mouse brain tissue lysate ; ; Lane 2.: Mouse liver tissue lysate

IF Description

ICC/IF analysis of GLUL in HeLa cells. The cell was stained with ATGA0474 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0474-IF1b.jpg}

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