Human Cyclophilin 40/PPID antibody

Cyclophilin D (also known as peptidylpropyl isomerase D, PPID) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression suppresses the apoptosis in cancer cells.

Product Specifications

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase D, Peptidylprolyl isomerase D, 40 kDa peptidyl-prolyl cis-trans isomerase, PPIase D, Cyclophilin-related protein, Rotamase D, CYP-40, CypD, Cyclophilin D

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPID (1-370aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1B8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PPID, ATGA0473-10 µg, ATGA0473-20 µg, ATGA0473-50 µg, ATGA0473-100 µg, ATGA0473-250 µg, ATGA0473-500 µg, ATGA0473-1 mg, ATGA0473-10, ATGA0473-20, ATGA0473-50, ATGA0473-100, ATGA0473-250, ATGA0473-500, ATGA0473-1

References & Citations

MachidaK., et al. (2006) J. Biol. Chem. 281 (20) : 14314-20.; ; Yokoi H., et al. (1996) Genomics. 35 (3) : 448-55.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_005029

Uniprot Accession Number

Q08752

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPID antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: MCF7 cell lysate; ; Lane 7.: SK-OV-3 cell lysate; ; Lane 8.: PC3 cell lysate

IF Description

ICC/IF analysis of PPID in Hep3B cells. The cell was stained with ATGA0473 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .