Human Chitinase 3-like 2/CHI3L2 antibody

CHI3L2, also known as chitinase-3-like protein 2, belongs to chitinase protein family. It is secreted by synovial fibroblasts, chondrocytes, and TGFb/IL4 stimulated monocyte derived macrophages. It has been proposed as a potential biomarker for osteoarthritis.

Product Specifications

Product Name Alternative

CHI3L2, CHIL2, YKL-39, YKL39

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CHI3L2 (27-390aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2B5

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Chitinase3-like2, ATGA0472-10 µg, ATGA0472-20 µg, ATGA0472-50 µg, ATGA0472-100 µg, ATGA0472-250 µg, ATGA0472-500 µg, ATGA0472-1 mg, ATGA0472-10, ATGA0472-20, ATGA0472-50, ATGA0472-100, ATGA0472-250, ATGA0472-500, ATGA0472-1

References & Citations

Hu B., et al. (1996) J. Biol. Chem. 271: 19415-19420.; ; Gratchev A., et al. (2008) Biomarker Insights. 3: 39-44.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Stem cell

NCBI Accession Number

NP_003991

Uniprot Accession Number

Q15782

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHI3L2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: HepG2 cell lysate; ; Lane 4.: LnCap cell lysate

FACS Description

Flow cytometry analysis of CHI3L2 in HeLa cells. The cell was stained with ATGA0472 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .