Human RAB7A antibody

Ras-related protein Rab-7a, also known as RAB7A, is a member of the Ras superfamily of small Rab GTPases. RAB7A associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes. RAB7A has also been shown to regulate growth receptor endocytic trafficking, degradation, maturation of phagosome and autophagic vacuoles.

Product Specifications

Product Name Alternative

Ras-related protein Rab-7a, RAB7, RAB7A member RAS oncogene family, CMT2B, Charcot-Marie-Tooth neuropathy 2B

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human RAB7A (1-207aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT10E4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Rab7a, ATGA0471-10 µg, ATGA0471-20 µg, ATGA0471-50 µg, ATGA0471-100 µg, ATGA0471-250 µg, ATGA0471-500 µg, ATGA0471-1 mg, ATGA0471-10, ATGA0471-20, ATGA0471-50, ATGA0471-100, ATGA0471-250, ATGA0471-500, ATGA0471-1

References & Citations

Feng Y., et al. (1995) J Cell Biol. 131: 1435-52.; ; Barbero P., et al. (2002) J Cell Biol. 156: 511-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Tags & Cell Markers

NCBI Accession Number

NP_004628

Uniprot Accession Number

P51149

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAB7A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: PC3 cell lysate; ; Lane 5.: 293T cell lysate; ; Lane 6.: SK-OV-3 cell lysate

FACS Description

Flow cytometry analysis of RAB7A in HeLa cells. The cell was stained with ATGA0471 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of RAB7A in HeLa cells. The cell was stained with ATGA0471 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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