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Human CTGF/CCN2 antibody

CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein) . CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.

Product Specifications

Product Name Alternative

Connective tissue growth factor, CCN2, NOV2, HCS24, IGFBP8

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CTGF (27-349 aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT18E7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CTGF, CCN2, Connective tissue growth factor, CCN2, NOV2, HCS24, IGFBP8, ATGA0468-10 µg, ATGA0468-20 µg, ATGA0468-50 µg, ATGA0468-100 µg, ATGA0468-250 µg, ATGA0468-500 µg, ATGA0468-1 mg, ATGA0468-10, ATGA0468-20, ATGA0468-50, ATGA0468-100, ATGA0468-250, ATGA0468-500, ATGA0468-1

References & Citations

Jun JI., et al. (2011) Nat Rev Drug Discov. 10 (12) : 945-63.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001892

Uniprot Accession Number

P29279

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTGF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: A549 cell lysate ; ; Lane 3.: U87MG cell lysate ; ; Lane 4.: A427 cell lysate

IF Description

ICC/IF analysis of CTGF in Hep3B cells. The cell was stained with ATGA0468 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0468-IF1b.jpg}
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