Human PGC1 alpha antibody

PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. This protein can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Also, induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner and involved in the integration of the circadian rhythms and energy metabolism. They required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.

Product Specifications

Product Name Alternative

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha, Ligand effect modulator 6, PPARGC1A, LEM6, PGC1, PGC1A, PPARGC1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPARGC1A (300-540aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT25C8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PGC1 a, ATGA0466-10 µg, ATGA0466-20 µg, ATGA0466-50 µg, ATGA0466-100 µg, ATGA0466-250 µg, ATGA0466-500 µg, ATGA0466-1 mg, ATGA0466-10, ATGA0466-20, ATGA0466-50, ATGA0466-100, ATGA0466-250, ATGA0466-500, ATGA0466-1

References & Citations

Lui Z., et al. (2016) Sci Rep. 6: 21382.; ; Li R., et al. (2016) Med Sci Monit. 22: 3229-3237.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_037393

Uniprot Accession Number

Q9UBK2

WB Description

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPARGC1A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: MCF7 cell lysate ; ; Lane 3.: HepG2 cell lysate ; ; Lane 4.: 293T cell lysate ; ; Lane 5.: Mouse brain tissue lysate

FACS Description

Flow cytometry analysis of PPARGC1A in HeLa cells. The cell was stained with ATGA0466 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of PPARGC1A in HeLa cells. The cell was stained with ATGA0466 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .