Human ACSF2 antibody

ACSF2 also known as Acyl-CoA synthetase family member 2. Acyl-CoA synthetases are a family of enzymes that catalyze the thioesterification of fatty acids with coenzymeA to form activated intermediates, which play a fundamental role in lipid metabolism and homeostasis of lipid-related processes. ACSF2 are required for complex lipid synthesis, energy production via beta-oxidation, protein acylation and fatty-acid dependent transcriptional regulation. ACSF2 are also necessary for fatty acid import into cells by the process of vectorial acylation.

Product Specifications

Product Name Alternative

Acyl-CoA synthetase family member 2 isoform 2, ACSMW, AVYV493

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACSF2 (42-615aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT18B4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACSF2, ATGA0464-10 µg, ATGA0464-20 µg, ATGA0464-50 µg, ATGA0464-100 µg, ATGA0464-250 µg, ATGA0464-500 µg, ATGA0464-1 mg, ATGA0464-10, ATGA0464-20, ATGA0464-50, ATGA0464-100, ATGA0464-250, ATGA0464-500, ATGA0464-1

References & Citations

Black PN., et al. (2007) Biochim Biophys Acta. 1771 (9) : 1246-53.; ; Watkins P.A., et al. (2007) J Lipid Res. 48 (12) : 2736-50.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_079425

Uniprot Accession Number

Q96CM8

WB Description

The HepG2 cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACSF2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of ACSF2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACSF2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)