Human CSK antibody

CSK is a tyrosine-protein kinase CSK that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

Product Specifications

Product Name Alternative

Tyrosine-protein kinase CSK, Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase CYL, Tyrosine protein kinase CSK

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CSK (1-450aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT7C6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CSK, ATGA0463-10 µg, ATGA0463-20 µg, ATGA0463-50 µg, ATGA0463-100 µg, ATGA0463-250 µg, ATGA0463-500 µg, ATGA0463-1 mg, ATGA0463-10, ATGA0463-20, ATGA0463-50, ATGA0463-100, ATGA0463-250, ATGA0463-500, ATGA0463-1

References & Citations

Braeuninger A., et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 10411-10415.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001120662

Uniprot Accession Number

P41240

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: CSK transfected 293T cell lysate

IF Description

ICC/IF analysis of CSK in HeLa cells. The cell was stained with ATGA0463 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .