Human Aminopeptidase P1/XPNPEP1 antibody

XPNPEP1 also known as Xaa-Pro aminopeptidase 1 isoform 1, is encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. This product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones.

Product Specifications

Product Name Alternative

Xaa-Pro aminopeptidase 1 isoform 1, APP1, SAMP, XPNPEP, XPNPEPL, XPNPEPL1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human XPNPEP1 (1-623aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT9C7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Aminopeptidase P1, XPNPEP1, ATGA0462-10 µg, ATGA0462-20 µg, ATGA0462-50 µg, ATGA0462-100 µg, ATGA0462-250 µg, ATGA0462-500 µg, ATGA0462-1 mg, ATGA0462-10, ATGA0462-20, ATGA0462-50, ATGA0462-100, ATGA0462-250, ATGA0462-500, ATGA0462-1

References & Citations

Cottrell G.S., et al. (2000) Biochemistry. 39: 15121-15128. ; ; Li X., et al. (2008) J. Biol. Chem. 283: 22858-22866.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_065116

Uniprot Accession Number

Q9NQW7

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPNPEP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: MCF7 cell lysate

IF Description

ICC/IF analysis of XPNPEP1 in HeLa cells. The cell was stained with ATGA0462 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .