Human NM23-H1/NME1 antibody

Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas.

Product Specifications

Product Name Alternative

NME/NM23 nucleoside diphosphate kinase 1, Nucleoside diphosphate kinase A, Granzyme A-activated Dnase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NDK A, NDP kinase A, NDPKA, NM23

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NME1 (1-152aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT5F4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NM23-H1, ATGA0461-10 µg, ATGA0461-20 µg, ATGA0461-50 µg, ATGA0461-100 µg, ATGA0461-250 µg, ATGA0461-500 µg, ATGA0461-1 mg, ATGA0461-10, ATGA0461-20, ATGA0461-50, ATGA0461-100, ATGA0461-250, ATGA0461-500, ATGA0461-1

References & Citations

Tee YT., et al. (2006) Taiwan J Obstet Gynecol. 45 (2) : 107-13.; ; Negroni A., et al. (2000) Cell Death Differ. 7 (9) : 843-50.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000260

Uniprot Accession Number

P15531

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : HeLa cell lysate; ; Lane 2 : A549 cell lysate; ; Lane 3 : Jurkat cell lysate; ; Lane 4 : HepG2 cell lysate; ; Lane 5 : MCF7 cell lysate; ; Lane 6 : PC3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : Recombinant Human NME1 protein; ; Lane 2 : Recombinant Human NME2 protein; ; Lane 3 : Recombinant Human NME3 protein; ; Lane 4 : Recombinant Human NME4 protein{ATGA0461-WB2.jpg}

IF Description

ICC/IF analysis of NME1 in HeLa cells. The cell was stained with ATGA0461 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of NME1 in A549 cells. The cell was stained with ATGA0461 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0461-IF2.jpg}