Human Leptin/OB antibody

Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation.

Product Specifications

Product Name Alternative

LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Leptin (22-167aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT7E10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Leptin, OB, ATGA0460-10 µg, ATGA0460-20 µg, ATGA0460-50 µg, ATGA0460-100 µg, ATGA0460-250 µg, ATGA0460-500 µg, ATGA0460-1 mg, ATGA0460-10, ATGA0460-20, ATGA0460-50, ATGA0460-100, ATGA0460-250, ATGA0460-500, ATGA0460-1

References & Citations

Anini Y., et al. (2003) Diabetes. 52 (2) : 252-259.; ; Jeong KJ., et al. (1999) Appl Environ Microbiol. 65 (7) : 3027-32.; ; Hyogo H., et al. (2002) J. Biol. Chem. 277 (37) : 34117-34124.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000221

Uniprot Accession Number

P41159

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Leptin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Leptin transfected 293T cell lysate

IF Description

ICC/IF analysis of Leptin in HeLa cells. The cell was stained with ATGA0460 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .