Human ACADS antibody

ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency) .

Product Specifications

Product Name Alternative

Acyl-Coenzyme A dehydrogenase, ACAD3, SCAD, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, short chain, Acyl-Coenzyme A dehydrogenase, short chain, AI196007, Bcd-1, Bcd1, Butyryl CoA dehydrogenase, EC 1.3.99.2, Short chain acyl CoA dehydrogenase, unsaturated acyl CoA reductase, Short-chain specific acyl-CoA dehydrogenase, mitochondrial.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACADS (25-412aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT8B10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACADS, Acyl-Coenzyme A dehydrogenase, ACAD3, SCAD, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, short chain, Acyl-Coenzyme A dehydrogenase, short chain, AI196007, Bcd-1, Bcd1, Butyryl CoA dehydrogenase, EC 1.3.99.2, Short chain acyl CoA dehydrogenase, unsaturated acyl CoA reductase, Short-chain specific acyl-CoA dehydrogenase, mitochondrial., ATGA0459-10 µg, ATGA0459-20 µg, ATGA0459-50 µg, ATGA0459-100 µg, ATGA0459-250 µg, ATGA0459-500 µg, ATGA0459-1 mg, ATGA0459-10, ATGA0459-20, ATGA0459-50, ATGA0459-100, ATGA0459-250, ATGA0459-500, ATGA0459-1

References & Citations

McAndrew RP., et al. (2008) J Biol Chem. 283 (14) : 9435-43. ; ; Tafti M., et al. (2003) Nat Genet. 34 (3) : 320-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

AAH25963

Uniprot Accession Number

P16219

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : HepG2 cell lysate ; ; Lane 2 : LnCap cell lysate

IF Description

ICC/IF analysis of ACADS in Hep3B cells. The cell was stained with ATGA0459 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of ACADS in A549 cells. The cell was stained with ATGA0459 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010398103791.jpg}