Human Serpin E1/PAI-1 antibody

Plasminogen activator inhibitor-1 (PAI-1), a member of the serine protease inhibitor (serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue.

Product Specifications

Product Name Alternative

Plasminogen activator inhibitor-1, PLANH1, SERPINE1, PAI1, PLANH1, Plasminogen activator inhibitor-1, Plasminogen activator inhibitor-1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PAI1 (24-402aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1C5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Serpin E1, PAI1, Plasminogen activator inhibitor-1, PLANH1, SERPINE1, PAI1, PLANH1, Plasminogen activator inhibitor-1, Plasminogen activator inhibitor-1, ATGA0458-10 µg, ATGA0458-20 µg, ATGA0458-50 µg, ATGA0458-100 µg, ATGA0458-250 µg, ATGA0458-500 µg, ATGA0458-1 mg, ATGA0458-10, ATGA0458-20, ATGA0458-50, ATGA0458-100, ATGA0458-250, ATGA0458-500, ATGA0458-1

References & Citations

Gorlatova NV., et al. (2007) J Biol Chem. 282 (12) : 9288-9296.; ; Renckens R., et al. (2006) J Immunol. 177 (11) : 8171-8176.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000593

Uniprot Accession Number

P05121

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PAI1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: PAI1 transfected 293T cell lysate

IF Description

ICC/IF analysis of PAI1 in HeLa cells. The cell was stained with ATGA0458 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .