Human RKIP/PEBP1 antibody

Phosphatidylethanolamine binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein (RKIP), is a member of the phosphatidylethanolamine-binding protein family and a serine protease inhibitor which inhibits thrombin, neuropsin. PEBP1 plays a pivotal modulatory role in several protein kinase signaling cascades. Protein kinase C (PKC) phosphorylates PEBP1, resulting in release of Raf-1 and activation of MEK and ERK. Expressed in many tissues, PEBP1 is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.

Product Specifications

Product Name Alternative

Phosphatidylethanolamine binding protein 1, PBP, Prostatic binding protein, RKIP, HCNP, HCNPpp, PEBP, Raf kinase inhibitory protein, Hippocampal cholinergic neurostimulating peptide

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PEBP1 (1-187aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT4B11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

RKIP, PEBP1, ATGA0456-10 µg, ATGA0456-20 µg, ATGA0456-50 µg, ATGA0456-100 µg, ATGA0456-250 µg, ATGA0456-500 µg, ATGA0456-1 mg, ATGA0456-10, ATGA0456-20, ATGA0456-50, ATGA0456-100, ATGA0456-250, ATGA0456-500, ATGA0456-1

References & Citations

Keller ET., et al. (2004) Biochem Pharmacol. 68 (6) : 1049-53. ; ; Park S., et al. (2005) Oncogene. 24 (21) : 3535-40.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002558

Uniprot Accession Number

P30086

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEBP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : HeLa cell lysate; ; Lane 2 : SKOV3 cell lysate; ; Lane 3 : A549 cell lysate; ; Lane 4 : HepG2 cell lysate; ; Lane 5 : MCF7 cell lysate; ; Lane 6 : K562 cell lysate; ; Lane 7 : LnCap cell lysate; ; Lane 8 : Ramos cell lysate

IF Description

ICC/IF analysis of PEBP1 in HeLa cells line, monoclonal anti-human PEBP1 antibody (1:100) stained with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .