Human BPGM antibody

Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2, 3-BPG concentration. It is responsible for the catalytic synthesis of 2, 3-Bisphosphoglycerate (2, 3-BPG) from 1, 3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active.

Product Specifications

Product Name Alternative

Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BPGM (1-259aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2E11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BPGM, Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192., ATGA0455-10 µg, ATGA0455-20 µg, ATGA0455-50 µg, ATGA0455-100 µg, ATGA0455-250 µg, ATGA0455-500 µg, ATGA0455-1 mg, ATGA0455-10, ATGA0455-20, ATGA0455-50, ATGA0455-100, ATGA0455-250, ATGA0455-500, ATGA0455-1

References & Citations

Pritlove DC., et al. (2006) Placenta. 27 (8) : 924-7. ; ; Wang Y., et al. (2006) J. Biol. Chem. 281 (51) : 39642-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001715

Uniprot Accession Number

P07738

WB Description

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BPGM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: BPGM transfected 293T cell lysate

FACS Description

Flow cytometry analysis of BPGM in HeLa cells. The cell was stained with ATGA0455 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of BPGM in HeLa cells. The cell was stained with ATGA0445 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .