Human HO-1/HMOX1/HSP32 antibody

Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Also this protein is known to play an important role in the regulation of cardiovascular function and its adaptive response to a variety of stressors.

Product Specifications

Product Name Alternative

HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Heme oxygenase1 (1-266aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1D6

Conjugation

Unconjugated

Applications

ELISA, WB, FACS, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HO1, HMOX1, HSP32, HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1., ATGA0454-10 µg, ATGA0454-20 µg, ATGA0454-50 µg, ATGA0454-100 µg, ATGA0454-250 µg, ATGA0454-500 µg, ATGA0454-1 mg, ATGA0454-10, ATGA0454-20, ATGA0454-50, ATGA0454-100, ATGA0454-250, ATGA0454-500, ATGA0454-1

References & Citations

Soares MP., et al. (2001) Immunol Rev.; ; Vareille M., et al. (2008) J Immunol. 180 (8) : 5720-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_002124

Uniprot Accession Number

P09601

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMOX antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: A549 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: HepG2 cell lysate ; ; Lane 5.: TF-1 cell lysate

FACS Description

Flow cytometry analysis of HMOX in HeLa cells. The cell was stained with ATGA0454 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of HMOX in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0454-IF1b.jpg}ICC/IF analysis of HMOX in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0454-IF2a.jpg} {ATGA0454-IF2b.jpg}